A hydroxyproline-containing glycopeptide released from the walls of sycamore tissue-culture cells by hydrazinolysis
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منابع مشابه
A hydroxyproline-containing glycopeptide released from the walls of sycamore tissue-culture cells by hydrazinolysis.
Degradation of the sycamore cell wall by hydrazinolysis yielded soluble glycopeptides. The peptide portion of the predominant glycopeptide was shown to be cyclic (4-trans-hydroxy-l-prolyl)-4-trans-hydroxy- l-proline. From this structure and the composition of the major glycopeptide it was deduced that only short oligosaccharides are attached to the hydroxyprolyl residues of the wall glycoprotei...
متن کاملGlycoprotein of the wall of sycamore tissue-culture cells.
1. A glycoprotein containing a large amount of hydroxyproline is present in the cell walls of sycamore callus cells. This protein is insoluble and remained in the alpha-cellulose when a mild separation procedure was used to obtain the polysaccharide fractions of the wall. The glycoprotein contained a high proportion of arabinose and galactose. 2. Soluble glycopeptides were prepared from the alp...
متن کاملDirect incorporation of hydroxyproline into protein of sycamore cells incubated at growth-inhibitory levels of hydroxyproline.
Free hydroxyproline inhibits the growth of animals1' I and plants.3-7 The mechanism of this inhibition is not known. Among the theoretically possible effects of hydroxyproline are: (1) reduction of protein synthesis by lowering proline incorporation, (2) interference with proline hydroxylation, and (3) direct incorporation of hydroxyproline resulting in nonfunctional proteins. Hydroxyproline re...
متن کاملEffect of trypsin on the cell surface proteins of hepatoma tissue culture cells. Characterization of a carbohydrate-rich glycopeptide released from a calcium binding membrane glycoprotein.
Concentrations of trypsin that bring about aggregation of hepatoma tissue culture (HTC) cells also release from the cell surface an Mr = 55,000 glycopeptide fragment. This glycopeptide fragment also accumulates in the medium, including serum-free medium, as a normal consequence of membrane protein turnover. The trypsin-released glycopeptide is labeled when cells are grown in the presence of fuc...
متن کاملComparative study of the oligosaccharides released from baby hamster kidney cells and their polyoma transformant by hydrazinolysis.
The asparagine-linked sugar chains of the membrane of baby hamster kidney cells and their polyoma transformant were quantitatively released as oligosaccharides by hydrazinolysis and labeled by NaB3H4 reduction. The radioactive oligosaccharides thus obtained were fractionated by paper electrophoresis. The neutral oligosaccharides of both cells were exclusively of high mannose type. The acidic ol...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1973
ISSN: 0264-6021
DOI: 10.1042/bj1350327